Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
ITPKB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of ITPKB using rabbit polyclonal ITPKB Antibody (R38) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ITPKB gene.| Product Name | ITPKB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inositol-trisphosphate 3-kinase B, Inositol 1,5-trisphosphate 3-kinase B, IP3 3-kinase B, IP3K B, InsP 3-kinase B, ITPKB |
| Product description | ITPKB regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.1) Dewaste, V., et al., Biochem. Biophys. Res. Commun. 291(2):400-405 (2002). |
| Immunogen | This ITPKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-54 amino acids from the N-terminal region of human ITPKB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ITPKB |
|---|---|
| Alternative Names | Inositol-trisphosphate 3-kinase B Inositol 1 5-trisphosphate 3-kinase B IP3 3-kinase B IP3K B InsP 3-kinase B ITPKB |
| Molecular Weight(MW) | 102 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
ITPKB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of ITPKB using rabbit polyclonal ITPKB Antibody (R38) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ITPKB gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
