Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ITPKC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of ITPKC Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | ITPKC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inositol-trisphosphate 3-kinase C, Inositol 1,5-trisphosphate 3-kinase C, IP3 3-kinase C, IP3K C, InsP 3-kinase C, ITPKC, IP3KC |
| Product description | ITPKC encodes a member of the inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] 3-kinase family of enzymes that catalyze the phosphorylation of inositol 1,4,5-trisphosphate to 1,3,4,5-tetrakisphosphate. The encoded protein is localized to the nucleus and cytoplasm and has both nuclear import and nuclear export activity. Single nucleotide polymorphisms in this gene are associated with Kawasaki disease.1) Chi,H. Hum. Mol. Genet. 19 (6), 1147-1151 (2010) |
| Immunogen | This ITPKC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human ITPKC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITPKC |
|---|---|
| Alternative Names | Inositol-trisphosphate 3-kinase C Inositol 1 5-trisphosphate 3-kinase C IP3 3-kinase C IP3K C InsP 3-kinase C ITPKC IP3KC |
| Molecular Weight(MW) | 75 kDa |
Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ITPKC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of ITPKC Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
