Application
Immunohistochemistry of IZUMO1 in human testis tissue with IZUMO1 antibody at 2.5 μg/mL.Application
Western blot analysis of IZUMO1 in human testis tissue lysate with IZUMO1 antibody at 1 μg/ml.| Product Name | IZUMO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Izumo sperm-egg fusion 1, IZUMO, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo |
| Product description | IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).1) Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140:3221-9. |
| Immunogen | IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1. The immunogen is located within amino acids 170 - 220 of IZUMO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IZUMO1 |
|---|---|
| Alternative Names | Izumo sperm-egg fusion 1 IZUMO Izumo sperm-egg fusion protein 1 Oocyte binding/fusion factor OBF Sperm-specific protein izumo |
| Molecular Weight(MW) | Predicted: 35, 39, 42 kDa Observed: 52 kDa |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms. |
Application
Immunohistochemistry of IZUMO1 in human testis tissue with IZUMO1 antibody at 2.5 μg/mL.
Application
Western blot analysis of IZUMO1 in human testis tissue lysate with IZUMO1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IZUMO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IZUMO1 antibody is supplied in PBS containing 0.02% sodium azide. |
