Application
Immunohistochemistry of JAGN1 in rat colon tissue with JAGN1 antibody at 2.5 μg/mL.Application
Western blot analysis of JAGN1 in MCF7 cell lysate with JAGN1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | JAGN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Jagunal homolog 1 |
| Product description | JAGN1 is the human ortholog of a gene originally identified in Drosophila melanogaster. It is a transmembrane protein that functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Individuals with mutations in the JAGN1 gene causes aberrant myeloid cell homeostasis and severe congenital neutropenia (SCN) (1). JAGN1-mutant granulocytes are characterized by ultrastructural defects, aberrant N-glycosylation of multiple proteins, and increased apoptosis (1). Mice lacking this gene could not mount and efficient neutrophil-dependent immune response to a human fungal pathogen (2).1) Boztug K, Jarvinen PM, Salzer E, et al. JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia. Nat. Genet. 2014; 46:1021-7. |
| Immunogen | JAGN1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAGN1. The immunogen is located within the last 50 amino acids of JAGN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JAGN1 |
|---|---|
| Alternative Names | Jagunal homolog 1 |
| Molecular Weight(MW) | Predicted: 20 kDa Observed: 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (95%) |
| Tissue Specificity | JAGN1 antibody is human, mouse and rat reactive. At least four isoforms of JAGN1 are known to exist; this antibody will detect all but isoform 2. |
Application
Immunohistochemistry of JAGN1 in rat colon tissue with JAGN1 antibody at 2.5 μg/mL.
Application
Western blot analysis of JAGN1 in MCF7 cell lysate with JAGN1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1411 - Mouse Colon Tissue Lysate |
|---|---|
| Application Notes | JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | JAGN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | JAGN1 antibody is supplied in PBS containing 0.02% sodium azide. |
