Application
Immunohistochemistry of JAK2 in mouse kidney tissue with JAK2 antibody at 5 μg/ml.Application
Western blot analysis of JAK2 in HeLa cell lysate with JAK2 antibody at 1 μg/ml.| Product Name | JAK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Janus kinase 2, JTK10, THCYT3 |
| Product description | JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2). JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3). Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4).1) Wilks AF, Harpur AG, Kurban RR, et al. Two novel protein-tyrosine kinases, each with a second phosphotransferase-related catalytic domain, define a new class of protein kinase. Mol. Cell Biol. 1991; 11:2057-66. |
| Immunogen | JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2. The immunogen is located within the last 50 amino acids of JAK2. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JAK2 |
|---|---|
| Alternative Names | Janus kinase 2 JTK10 THCYT3 |
| Molecular Weight(MW) | Predicted: 125 kDa Observed: 125 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
| Tissue Specificity | JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins. |
Application
Immunohistochemistry of JAK2 in mouse kidney tissue with JAK2 antibody at 5 μg/ml.
Application
Western blot analysis of JAK2 in HeLa cell lysate with JAK2 antibody at 1 μg/ml.| Positive Control | 3T3 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | JAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | JAK2 antibody is supplied in PBS containing 0.02% sodium azide. |
