Application
Western blot analysis of anti-JHDM1a/FBXL11 Antibody pre-incubated with(right lane) and without(left lane) blocking peptide in Jurkat cell line lysate.| Product Name | JHDM1a/FBXL11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lysine-specific demethylase 2A, CXXC-type zinc finger protein 8, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, F-box/LRR-repeat protein 11, JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-3 |
| Product description | JHDM1a/FBXL11 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. JHDM1a/FBXL11 belongs to the Fbls class and, in addition to an F-box, contains at least 6 highly degenerated leucine-rich repeats.1) Tsukada,Y.,Nature 439 (7078), 811-816 (2006) |
| Immunogen | This JHDM1a/FBXL11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 867-896 amino acids from the C-terminal region of human JHDM1a/FBXL11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KDM2A |
|---|---|
| Alternative Names | Lysine-specific demethylase 2A CXXC-type zinc finger protein 8 F-box and leucine-rich repeat protein 11 F-box protein FBL7 F-box protein Lilina F-box/LRR-repeat protein 11 JmjC domain-containing histone demethylation protein 1A [Histone-H3]-lysine-3 |
| Molecular Weight(MW) | 133 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of anti-JHDM1a/FBXL11 Antibody pre-incubated with(right lane) and without(left lane) blocking peptide in Jurkat cell line lysate.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
