Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of JHDM1D Antibody in 293 cell line lysates (35ug/lane)Application
JHDM1D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | JHDM1D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lysine-specific demethylase 7A, 11411-, JmjC domain-containing histone demethylation protein 1D, Lysine-specific demethylase 7, KDM7A, JHDM1D, KDM7, KIAA1718 |
| Product description | JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3. |
| Immunogen | This JHDM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-316 amino acids from the Central region of human JHDM1D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDM7A |
|---|---|
| Alternative Names | Lysine-specific demethylase 7A 11411- JmjC domain-containing histone demethylation protein 1D Lysine-specific demethylase 7 KDM7A JHDM1D KDM7 KIAA1718 |
| Molecular Weight(MW) | 107 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of JHDM1D Antibody in 293 cell line lysates (35ug/lane)
Application
JHDM1D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
