Application
Immunohistochemistry of JMJD1C in rat liver tissue with JMJD1C antibody at 2.5 μg/mL.Application
Western blot analysis of JMJD1C in human liver tissue lysate with JMJD1C antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of JMJD1C in rat liver tissue with JMJD1C antibody at 20 μg/mL.| Product Name | JMJD1C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | JMJD1C Antibody: The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist.1) Katoh M and Katoh M. Identification of TRIP8 gene in silico. Int. J. Mol. Med.2003; 12:817-21. |
| Immunogen | JMJD1C antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human JMJD1C. The immunogen is located within amino acids 380 - 430 of JMJD1C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| JMJD1C antibody can be used for detection of JMJD1C by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JMJD1C |
|---|---|
| Alternative Names | JMJD1C Antibody: TRIP8 JHDM2C KIAA1380 TRIP8 Probable JmjC domain-containing histone demethylation protein 2C Jumonji domain-containing protein 1C TR-interacting protein 8 |
| Tissue Specificity | This antibody will not cross-react with JMJD1A or JMJD1B. |
Application
Immunohistochemistry of JMJD1C in rat liver tissue with JMJD1C antibody at 2.5 μg/mL.
Application
Western blot analysis of JMJD1C in human liver tissue lysate with JMJD1C antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of JMJD1C in rat liver tissue with JMJD1C antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
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| Application Notes | JMJD1C antibody can be used for detection of JMJD1C by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | JMJD1C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JMJD1C Antibody is supplied in PBS containing 0.02% sodium azide. |
