Application
Western blot analysis of JMJD2B in A20 cell lysate with JMJD2B antibody at (A) 1 and (B) 2 μg/mL.| Product Name | JMJD2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JMJD2B Antibody: JMJD2B, TDRD14B, JHDM3B, JMJD2B, KIAA0876, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B |
| Product description | JMJD2B Antibody: Members of the Jumonji domain 2 (JMJD2) family contain a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. Like other members of the JMJD1 and JMJD2 families, JMJD2B is a histone demethylase, but is reported to be a very poor enzyme. Similar to JMJD1A, JMJD2B is a transcriptional target of the hypoxia-inducible factor (HIF), and hypoxic cells express elevated levels of the JMJD2B mRNA and protein. Both JMJD1A and JMJD2B retain their histone demethylase activity in hypoxic conditions, suggesting that this activity of JMJD2B might play a role in the hypoxic gene expression program.1) Katoh M and Katoh M. Identification and characterization of JMJD2 family genes in silico. Int. J. Oncol.2004; 24:1623-8. |
| Immunogen | JMJD2B antibody was raised against a 16 amino acid synthetic peptide from near the center of human JMJD2B. The immunogen is located within amino acids 360 - 410 of JMJD2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| JMJD2B antibody can be used for detection of JMJD2B by Western blot at 1 - 2 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDM4B |
|---|---|
| Alternative Names | JMJD2B Antibody: JMJD2B TDRD14B JHDM3B JMJD2B KIAA0876 Lysine-specific demethylase 4B JmjC domain-containing histone demethylation protein 3B |
Application
Western blot analysis of JMJD2B in A20 cell lysate with JMJD2B antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
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| Application Notes | JMJD2B antibody can be used for detection of JMJD2B by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | JMJD2B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JMJD2B Antibody is supplied in PBS containing 0.02% sodium azide. |
