Application
Western blot analysis of anti-JMJD2D Pab in Hela cell line lysate| Product Name | JMJD2D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lysine-specific demethylase 4D, 11411-, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D, KDM4D, JHDM3D, JMJD2D |
| Product description | Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.1) Katoh, M., et al., Int. J. Oncol. 24(6):1623-1628 (2004). |
| Immunogen | This JMJD2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-516 amino acids from the C-terminal region of human JMJD2D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KDM4D |
|---|---|
| Alternative Names | Lysine-specific demethylase 4D 11411- JmjC domain-containing histone demethylation protein 3D Jumonji domain-containing protein 2D KDM4D JHDM3D JMJD2D |
| Molecular Weight(MW) | 59 kDa |
Application
Western blot analysis of anti-JMJD2D Pab in Hela cell line lysate| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
