Application
Immunohistochemistry of JMJD6 in rat brain tissue with JMJD6 antibody at 2.5 μg/mL.Application
Immunofluorescence of JMJD6 in rat brain tissue with JMJD6 antibody at 20 μg/mL.Application
Western blot analysis of JMJD6 in human brain tissue lysate with JMJD6 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | JMJD6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JMJD6 Antibody: PSR, PTDSR, PTDSR1, KIAA0585, Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, Histone arginine demethylase JMJD6, Protein PTDSR |
| Product description | JMJD6 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. JMJD6 was initially identified as a membrane protein that participates in phagocytosis but recent studies have shown that it has other functions when it is expressed in the cytosol and nucleus. JMJD6 is thought to play important roles in regulation of development and differentiation as knockdown experiments in mice resulted in neonatal lethality with severe defects in the morphology of numerous organs. JMJD6 also can catalyze the lysyl-hydroxylation of U2AF65, a protein involved with RNA splicing, suggesting that some of the functions attributed to JMJD6 may be due to its regulatory activity of RNA splicing.1) Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59. |
| Immunogen | JMJD6 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human JMJD6. The immunogen is located within amino acids 70 - 120 of JMJD6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| JMJD6 antibody can be used for detection of JMJD6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JMJD6 |
|---|---|
| Alternative Names | JMJD6 Antibody: PSR PTDSR PTDSR1 KIAA0585 Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 Histone arginine demethylase JMJD6 Protein PTDSR |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%) |
Application
Immunohistochemistry of JMJD6 in rat brain tissue with JMJD6 antibody at 2.5 μg/mL.
Application
Immunofluorescence of JMJD6 in rat brain tissue with JMJD6 antibody at 20 μg/mL.
Application
Western blot analysis of JMJD6 in human brain tissue lysate with JMJD6 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | JMJD6 antibody can be used for detection of JMJD6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | JMJD6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JMJD6 Antibody is supplied in PBS containing 0.02% sodium azide. |
