WB
Western blot analysis of JMJD6 on different lysates with Rabbit anti-JMJD6 antibody at 1/5,000 dilution. Lane 1: 293T cell lysate, Lane 2: PANC-1 cell lysate, Lane 3: HeLa cell lysate, Lane 4: SH-SY5Y cell lysate, Lane 5: COS-1 cell lysate, Lane 6: Neuro-2a cell lysate, Lane 7: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling JMJD6 with Rabbit anti-JMJD6 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JMJD6 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of PC-12 cells labeling JMJD6. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
JMJD6 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-JMJD6 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-JMJD6 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-JMJD6 antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-JMJD6 antibody in HeLa cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 minutes.ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with JMJD6 or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Product Name | JMJD6 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human JMJD6 aa 1-360. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPFCICC/IFIHCIPWB |
| WB:1:5000 IHC:1:8000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample ChIP:Use 5 μg for 25 μg of chromatin. |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | JMJD6 |
|---|---|
| Gene Synonyms | PSR PTDSR PTDSR1 |
| Gene Full Name | jumonji domain containing 6, arginine demethylase and lysine hydroxylase |
| Gene Summary | This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Apoptotic cell clearance receptor antibody Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody Histone arginine demethylase JMJD6 antibody JmjC domain-containing protein 6 antibody JMJD 6 antibody JMJD6 antibody JMJD6_HUMAN antibody Jumonji domain containing 6 antibody Jumonji domain-containing protein 6 antibody KIAA0585 antibody Apoptotic cell clearance receptor antibody Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody Histone arginine demethylase JMJD6 antibody JmjC domain-containing protein 6 antibody JMJD 6 antibody JMJD6 antibody JMJD6_HUMAN antibody Jumonji domain containing 6 antibody Jumonji domain-containing protein 6 antibody KIAA0585 antibody Lysyl-hydroxylase JMJD6 antibody Peptide-lysine 5-dioxygenase JMJD6 antibody Phosphatidylserine receptor antibody Protein PTDSR antibody PSR antibody PTDSR 1 antibody PTDSR antibody PTDSR1 antibody |
| Molecular Weight(MW) | 46kDa(Observed band size: 50/110-260kDa) |
| Cellular Localization | Nucleus, nucleoplasm, nucleolus, Cytoplasm. |
WB
Western blot analysis of JMJD6 on different lysates with Rabbit anti-JMJD6 antibody at 1/5,000 dilution. Lane 1: 293T cell lysate, Lane 2: PANC-1 cell lysate, Lane 3: HeLa cell lysate, Lane 4: SH-SY5Y cell lysate, Lane 5: COS-1 cell lysate, Lane 6: Neuro-2a cell lysate, Lane 7: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling JMJD6 with Rabbit anti-JMJD6 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JMJD6 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of PC-12 cells labeling JMJD6. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
JMJD6 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-JMJD6 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-JMJD6 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-JMJD6 antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-JMJD6 antibody in HeLa cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 minutes.
ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with JMJD6 or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Application Notes | WB:1:5000 IHC:1:8000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample ChIP:Use 5 μg for 25 μg of chromatin. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
