Application
Immunofluorescence staining of methanol-fixed HeLa cells using JNK1/JNK2 (phospho-Thr183/Tyr185).Application
Western blot analysis of lysed extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2 (phospho-Thr183/Tyr185).| Product Name | JNK1 / JNK2 (phospho Thr183 / Tyr185) Antibody |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | JNK, JNK1, PRKM8, SAPK1, JNK-46, JNK1A2, SAPK1c, JNK21B1/2, FLT, FRT, VEGFR1, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1 |
| Product description | Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it./Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity1) Davis, R. J. (1999) Biochem Soc Symp 64, 1-12. Ichijo, H. (1999) Oncogene 18, 6087-93. Kyriakis, J. M. and Avruch, J. (2001) Physiol Rev 81, 807-69. |
| Immunogen | JNK1/JNK2 (phospho-Thr183/Tyr185) antibody was raised against a peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T (p) -P-Y (p) - V - V ) derived from Human JNK1/JNK2. |
| Modification | p-Thr183 / Tyr185 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK8 MAPK9 MAPK10 |
|---|---|
| Alternative Names | JNK JNK1 PRKM8 SAPK1 JNK-46 JNK1A2 SAPK1c JNK21B1/2 FLT FRT VEGFR1 Stress-activated protein kinase JNK1 c-Jun N-terminal kinase 1 |
| Molecular Weight(MW) | 46, 54 kDa |
| Tissue Specificity | This antibody detects endogenous level of JNK1/JNK2 only when phosphorylated at Thr183/Tyr185. |
| Entrez Gene | 5599 / 5601 / 5602 |
|---|---|
| Protein Accession | NP_002741.1 / NP_001128516.1 / NP_002744.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using JNK1/JNK2 (phospho-Thr183/Tyr185).
Application
Western blot analysis of lysed extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2 (phospho-Thr183/Tyr185).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
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| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
