Application
Western blot analysis of JPH2 in 293 cell lysate with JPH2 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | JPH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JPH2 Antibody: JP4, JPHL1, KIAA1831, Junctophilin-4, Junctophilin-like 1 protein, JP-4 |
| Product description | JPH2 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH2-null mice died of embryonic cardiac arrest and human patients with mutations in the JPH2 gene showed hypertrophic cardiomyopathy, demonstrating the importance of this protein. Multiple isoforms of JPH2 are known to exist.1) Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell.2000; 6:11-22. |
| Immunogen | JPH2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human JPH2. The immunogen is located within amino acids 510 - 560 of JPH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| JPH2 antibody can be used for detection of JPH2 by Western blot at 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JPH4 |
|---|---|
| Alternative Names | JPH2 Antibody: JP4 JPHL1 KIAA1831 Junctophilin-4 Junctophilin-like 1 protein JP-4 |
Application
Western blot analysis of JPH2 in 293 cell lysate with JPH2 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | JPH2 antibody can be used for detection of JPH2 by Western blot at 2 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | JPH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JPH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
