Application
Western blot analysis of JPH4 in mouse brain tissue lysate with JPH4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | JPH4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JPH4 Antibody: JP4, JPHL1, KIAA1831, Junctophilin-4, Junctophilin-like 1 protein, JP-4 |
| Product description | JPH4 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain. In the CNS, both JPH3 and JPH4 are expressed throughout neural sites and contribute to the subsurface cistern formation in neurons. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions.1) Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell.2000; 6:11-22. |
| Immunogen | JPH4 antibody was raised against a 17 amino acid synthetic peptide near the center of human JPH4. The immunogen is located within amino acids 410 - 460 of JPH4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| JPH4 antibody can be used for detection of JPH4 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JPH4 |
|---|---|
| Alternative Names | JPH4 Antibody: JP4 JPHL1 KIAA1831 Junctophilin-4 Junctophilin-like 1 protein JP-4 |
Application
Western blot analysis of JPH4 in mouse brain tissue lysate with JPH4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | JPH4 antibody can be used for detection of JPH4 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | JPH4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JPH4 Antibody is supplied in PBS containing 0.02% sodium azide. |
