Application
Immunofluorescence of JPH3 in Human Brain cells with JPH3 antibody at 20 μg/mL.Application
Immunohistochemistry of JPH3 in human brain tissue with JPH3 antibody at 2.5 μg/mL.Application
Western blot analysis of JPH3 in Daudi cell lysate with JPH3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | JPH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JPH3 Antibody: JP3, HDL2, JP-3, TNRC22, CAGL237, JP3, Junctophilin-3, Junctophilin type 3 |
| Product description | JPH3 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain. In the CNS, both JPH3 and JPH4 are expressed throughout neural sites and contribute to the subsurface cistern formation in neurons. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions. A repeat expansion in JPH3 is associated with Huntington disease-like 2. At least two isoforms of JPH3 are known to exist.1) Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell.2000; 6:11-22. |
| Immunogen | JPH3 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human JPH3. The immunogen is located within amino acids 540 - 590 of JPH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| JPH3 antibody can be used for detection of JPH3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JPH3 |
|---|---|
| Alternative Names | JPH3 Antibody: JP3 HDL2 JP-3 TNRC22 CAGL237 JP3 Junctophilin-3 Junctophilin type 3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of JPH3 in Human Brain cells with JPH3 antibody at 20 μg/mL.
Application
Immunohistochemistry of JPH3 in human brain tissue with JPH3 antibody at 2.5 μg/mL.
Application
Western blot analysis of JPH3 in Daudi cell lysate with JPH3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | JPH3 antibody can be used for detection of JPH3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | JPH3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JPH3 Antibody is supplied in PBS containing 0.02% sodium azide. |
