Application
Immunocytochemistry of KappaB-ras1 in RAW264.7 cells with KappaB-ras1 antibody at 1 μg/mL.Application
Western blot analysis of KappaB ras1 in RAW264.7 cell lysate with KappaB ras1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | KappaB ras1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KappaB ras1 Antibody: KBRAS1, kappaB-Ras1, KBRAS1, NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1 |
| Product description | KappaB ras1 Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody is specific for kappaB-ras1 and has no cross-reactivity to kappaB-ras2.1) Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73. |
| Immunogen | KappaB ras1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human KappaB ras1. The immunogen is located within the last 50 amino acids of KappaB ras1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| KappaB ras1 antibody can be used for detection of KappaB ras1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NKIRAS1 |
|---|---|
| Alternative Names | KappaB ras1 Antibody: KBRAS1 kappaB-Ras1 KBRAS1 NF-kappa-B inhibitor-interacting Ras-like protein 1 I-kappa-B-interacting Ras-like protein 1 Kappa B-Ras protein 1 |
| Sequence Similarities | 2) Cat. No. 17-203 - RAW 264.7 Cell Slide |
Application
Immunocytochemistry of KappaB-ras1 in RAW264.7 cells with KappaB-ras1 antibody at 1 μg/mL.
Application
Western blot analysis of KappaB ras1 in RAW264.7 cell lysate with KappaB ras1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
|---|---|
| Application Notes | KappaB ras1 antibody can be used for detection of KappaB ras1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KappaB ras1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KappaB ras1 Antibody is supplied in PBS containing 0.02% sodium azide. |
