Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.| Product Name | KARS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KARS, KRS, KARS1, KARS2, CMTRIB, DFNB89 |
| Product description | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.1) Halwani, R., (2004) J. Virol. 78 (14), 7553-7564. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KARS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| KARS antibody can be used for detection of KARS by ELISA at 1:312500. KARS antibody can be used for detection of KARS by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KARS |
|---|---|
| Alternative Names | KARS KRS KARS1 KARS2 CMTRIB DFNB89 |
| Molecular Weight(MW) | 66 kDa |
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | KARS antibody can be used for detection of KARS by ELISA at 1:312500. KARS antibody can be used for detection of KARS by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KARS antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
