Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in WB on Mouse renal epithelial at: 1:500 (Lane 1: 30ug mouse renal epithelial lysate, Lane 2: 30ug mouse renal epithelial lysate, Lane 3: 30ug mouse renal epithelial lysate).| Product Name | KCNJ1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KCNJ1 has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for KCNJ1.Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis.Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.1) Tobin, M.D., (2008) Hypertension 51 (6), 1658-1664. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KCNJ1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,WB |
| KCNJ1 antibody can be used for detection of KCNJ1 by ELISA at 1:312500. KCNJ1 antibody can be used for detection of KCNJ1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNJ1 |
|---|---|
| Alternative Names | KCNJ1 KIR1.1 ROMK ROMK1 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on Mouse renal epithelial at: 1:500 (Lane 1: 30ug mouse renal epithelial lysate, Lane 2: 30ug mouse renal epithelial lysate, Lane 3: 30ug mouse renal epithelial lysate).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | KCNJ1 antibody can be used for detection of KCNJ1 by ELISA at 1:312500. KCNJ1 antibody can be used for detection of KCNJ1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KCNJ1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
