Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of KCNJ2 Antibody in mouse lung tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KCNJ2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.| Product Name | KCNJ2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inward rectifier potassium channel 2, Cardiac inward rectifier potassium channel, Inward rectifier K(+) channel Kir21, IRK-1, hIRK1, Potassium channel, inwardly rectifying subfamily J member 2, KCNJ2, IRK1 |
| Product description | Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. This protein is an integral membrane protein and inward-rectifier type potassium channel. This protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is racterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features. |
| Immunogen | This KCNJ2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-427 amino acids from the C-terminal region of human KCNJ2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNJ2 |
|---|---|
| Alternative Names | Inward rectifier potassium channel 2 Cardiac inward rectifier potassium channel Inward rectifier K(+) channel Kir21 IRK-1 hIRK1 Potassium channel inwardly rectifying subfamily J member 2 KCNJ2 IRK1 |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of KCNJ2 Antibody in mouse lung tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KCNJ2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
