Application
Immunohistochemistry of KCNK1 in mouse brain tissue with KCNK1 antibody at 5 μg/ml.Application
Immunofluorescence of KCNK1 in mouse brain tissue with KCNK1 antibody at 20 μg/ml.Application
Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 μg/ml.| Product Name | KCNK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The potassium channel, subfamily K, member 1 (KCNK1), is member of the superfamily of potassium channel proteins containing two pore-forming P domains (1,2). KCNK1 is a ubiquitous human weakly inward rectifying K+ channel that is widely expressed in human tissues, and particularly abundant in brain and heart (1). Both KCNK1 and the related protein KCNK2 contribute significantly to the passive conductance of hippocampal astrocytes (3).1) Lesage F, Guillemare E, Fink M, et al. TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure. EMBO J. 1996; 15:1004-11. |
| Immunogen | KCNK1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KCNK1. The immunogen is located within amino acids 220 - 270 of KCNK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| KCNK1 antibody can be used for detection of KCNK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNK1 |
|---|---|
| Alternative Names | Potassium channel subfamily K member 1 DPK HOHO K2P1 K2p1.1 KCNO1 TWIK-1 TWIK1 |
| Molecular Weight(MW) | Predicted: 37 kDa Observed: 35 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (100%) |
| Tissue Specificity | KCNK1 antibody is human, mouse and rat reactive. KCNK1 antibody is predicted to not cross-react with other KCNK proteins. |
Application
Immunohistochemistry of KCNK1 in mouse brain tissue with KCNK1 antibody at 5 μg/ml.
Application
Immunofluorescence of KCNK1 in mouse brain tissue with KCNK1 antibody at 20 μg/ml.
Application
Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | KCNK1 antibody can be used for detection of KCNK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KCNK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | KCNK1 antibody is supplied in PBS containing 0.02% sodium azide. |
