Application
Immunofluorescence of KCNK12 in mouse brain tissue with KCNK12 antibody at 20 μg/mL.Application
Immunohistochemistry of KCNK12 in mouse brain tissue with KCNK12 antibody at 5 μg/mL.Application
Western blot analysis of KCNK12 in rat brain tissue lysate with KCNK12 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | KCNK12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KCNK12 Antibody: KCNK13 and KCNK12 (also known as THIK1 and 2) are the first two members of a novel two pore-forming P domains K+ channels subfamily. The pore loop domain, a highly conserved region common to all potassium channels, is involved in determining potassium ion selectivity. Members of this family are all characterized by four transmembrane domains and may function to help influence the resting membrane potential of cells. KCNK12 is expressed mainly in the brain and lung, but also observed in the kidneys, heart and skeletal muscle. KCNK12 is closely related to KCNK13 (58% identity at the amino acid level), but could not yet been functionally expressed in vitro and may require other proteins to become active.1) Rajan S, Wischmeyer E, Karschin C, et al. THIK-1 and THIK-2, a novel subfamily of tandem pore domain K+ channels. J. Biol. Chem. 2001; 276:7302-11 |
| Immunogen | KCNK12 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human KCNK12. The immunogen is located within amino acids 320 - 370 of KCNK12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| KCNK12 antibody can be used for detection of KCNK12 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNK12 |
|---|---|
| Alternative Names | KCNK12 Antibody: THIK2 THIK-2 K2p12.1 Potassium channel subfamily K member 12 Tandem pore domain halothane-inhibited potassium channel 2 |
| Tissue Specificity | KCNK12 antibody is predicted to not cross-react with other KCNK protein family members. |
Application
Immunofluorescence of KCNK12 in mouse brain tissue with KCNK12 antibody at 20 μg/mL.
Application
Immunohistochemistry of KCNK12 in mouse brain tissue with KCNK12 antibody at 5 μg/mL.
Application
Western blot analysis of KCNK12 in rat brain tissue lysate with KCNK12 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | KCNK12 antibody can be used for detection of KCNK12 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KCNK12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KCNK12 Antibody is supplied in PBS containing 0.02% sodium azide. |
