Application
Flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with KCTD12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of KCTD12 Antibody in T47D cell line lysates (35ug/lane)| Product Name | KCTD12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BTB/POZ domain-containing protein KCTD12, Pfetin, Predominantly fetal expressed T1 domain, KCTD12, C13orf2, KIAA1778, PFET1 |
| Product description | 1) Drenos,F., et.al., Hum. Mol. Genet. 18 (12), 2305-2316 (2009) Suehara,Y., et.al., Clin. Cancer Res. 14 (6), 1707-1717 (2008) Cauchi,S., et.al., J. Mol. Med. 86 (3), 341-348 (2008) |
| Immunogen | This KCTD12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the Central region of human KCTD12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCTD12 |
|---|---|
| Alternative Names | BTB/POZ domain-containing protein KCTD12 Pfetin Predominantly fetal expressed T1 domain KCTD12 C13orf2 KIAA1778 PFET1 |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with KCTD12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of KCTD12 Antibody in T47D cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
