Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (Phospho-Ser33).Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with EGF using Keratin 18 (Phospho-Ser33).| Product Name | Keratin 18 (phospho Ser33) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.1) Ramaekers, F. C. and Bosman, F. T. (2004) J. Pathol. 204, 351-354. Chang, L. and Goldman, R. D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613. Moll, R. et al. (1982) Cell 31, 11-24. |
| Immunogen | Keratin 18 (Phospho-Ser33) antibody was raised against a peptide sequence around phosphorylation site of serine 33 (A-A-S (p) -V-Y) derived from Human Keratin 18 (CK18). |
| Modification | p-Ser33 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT18 |
|---|---|
| Alternative Names | K18 CYK18 CBL2 RNF55 CK 18 CK18 Cytokeratin endo B K18 |
| Molecular Weight(MW) | 46 kDa |
| Tissue Specificity | This antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33. |
| Entrez Gene | 3875 |
|---|---|
| Protein Accession | NP_000215.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (Phospho-Ser33).
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with EGF using Keratin 18 (Phospho-Ser33).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
