Application
Antibody is used in Western blot to detect KHK in mouse liver tissue lysate (Lane 1) and 293 cell lysate (Lane 2).Application
Western blot analysis of KHK using rabbit polyclonal Ketohexokinase (KHK) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KHK gene (Lane 2).| Product Name | Ketohexokinase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ketohexokinase (KHK), or fructokinase, catalyzes conversion of fructose to fructose-1-phosphate. Splice variant 1 is the highly active form found in liver, renal cortex, and small intestine, while splice variant 2 is the lower activity form found in most other tissues. KHK, like glucokinase (GCK) and glucokinase regulator (GCKR), is present in both liver and pancreatic islets. The inhibition of GCK by GCKR is blocked by binding of fructose-1-phosphate to GCKR. The chromosomal proximity of the metabolically connected GCKR and KHK genes has a genetic linkage in type 2 diabetes. Fructosuria, or hepatic fructokinase deficiency, is a benign, asymptomatic defect of intermediary metabolism associated with heterozygosity for G50R and A43T mutations in KHK.1) Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). |
| Immunogen | This Ketohexokinase (KHK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human Ketohexokinase (KHK). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | KHK |
|---|---|
| Alternative Names | Ketohexokinase Hepatic fructokinase KHK |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Antibody is used in Western blot to detect KHK in mouse liver tissue lysate (Lane 1) and 293 cell lysate (Lane 2).
Application
Western blot analysis of KHK using rabbit polyclonal Ketohexokinase (KHK) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KHK gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
