KHK is a ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose.KHK encodes the gene ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The splice variant presented encodes the highly active form found in liver, renal cortex, and small intestine, while the alternate variant encodes the lower activity form found in most other tissues.1) Hwa, J.S., (2006) Proteomics 6 (3), 1077-1084.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KHK.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
IHCWB
KHK antibody can be used for detection of KHK by ELISA at 1:312500. KHK antibody can be used for detection of KHK by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Positive Control
1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Application Notes
KHK antibody can be used for detection of KHK by ELISA at 1:312500. KHK antibody can be used for detection of KHK by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KHK antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.