KIAA0317 contains 1 filamin repeat and 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. The exact function of KIAA0317 remains unknown.1) Heilig, R., (2003) Nature 421 (6923), 601-607.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KIAA0317.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
KIAA0317 antibody can be used for detection of KIAA0317 by ELISA at 1:62500. KIAA0317 antibody can be used for detection of KIAA0317 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Application Notes
KIAA0317 antibody can be used for detection of KIAA0317 by ELISA at 1:62500. KIAA0317 antibody can be used for detection of KIAA0317 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KIAA0317 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.