Application
Immunofluorescence of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 20 μg/ml.Application
Immunohistochemistry of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 5 μg/ml.Application
Western blot analysis of KIRREL3 in mouse kidney tissue lysate with KIRREL3 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | KIRREL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KIRREL3, also known as nephrin-like protein 2, is a type I transmembrane protein belonging to a family of three podocin interacting proteins and the immunoglobulin superfamily (1). KIRREL3 is involved in the regulation of both glomerular and neural development (2), and more specifically, the nucleogenesis of the pontine nuclei in the developing hindbrain (3). KIRREL3 has also been shown to interact with the synaptic scaffold protein calmodulin-associated serine/threonine kinase (CASK) in neuronal cells (4).1) Sellin L, Huber TB, Gerke P, et al. NEPH1 defines a novel family of podocin interacting proteins. FASEB J. 2003; 17:115-7. |
| Immunogen | KIRREL3 antibody was raised against a 19 amino acid peptide near the amino terminus of human KIRREL3. The immunogen is located within amino acids 20 - 70 of KIRREL3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| KIRREL3 antibody can be used for detection of KIRREL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIRREL3 |
|---|---|
| Alternative Names | Kin of irregular chiasm-like protein 3 Kin of IRRE-like protein 3 MRD4 KIRRE NEPH2 PRO4502 Nephrin-like protein 2 |
| Molecular Weight(MW) | Predicted: 64, 84 kDa Observed: 60 kDa |
| Tissue Specificity | KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family. |
Application
Immunofluorescence of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 20 μg/ml.
Application
Immunohistochemistry of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 5 μg/ml.
Application
Western blot analysis of KIRREL3 in mouse kidney tissue lysate with KIRREL3 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | KIRREL3 antibody can be used for detection of KIRREL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KIRREL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | KIRREL3 antibody is supplied in PBS containing 0.02% sodium azide. |
