Application
Antibody is used in Western blot to detect KIST in K562 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).Application
Western Blot at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: A-673 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | KIST Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KIST, a member of the Ser/Thr protein kinase family, is a pyruvate kinase that catalyzes formation of phosphoenolpyruvate from pyruvate and ATP. A role for the primarily nuclear KIST protein in mediation of cellular metabolism has been postulated based on the interaction identified with thyroid hormone. KIST is widely expressed, with highest abundance in skeletal muscle, kidney, placenta and peripheral blood leukocytes. Upon serum stimulation, KIST phosphorylates CDKN1B/p27Kip1, thereby regulating the subcellular location of CDKN1B and cell cycle progression in the G1 phase. KIST, which contains one RNA recognition motif domain, has been proposed to partipate in trafficking and processing of RNA. KIST binds to Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells.1) Bieche, I., et al., Brain Res. Mol. Brain Res. 114(1):55-64 (2003). |
| Immunogen | This KIST (KIS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the C-terminal region of human KIST (KIS). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | UHMK1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase Kist Kinase interacting with stathmin PAM COOH-terminal interactor protein 2 P-CIP2 U2AF homology motif kinase 1 UHMK1 KIS KIST |
| Molecular Weight(MW) | 47 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Antibody is used in Western blot to detect KIST in K562 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Application
Western Blot at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: A-673 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
