Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).| Product Name | KIT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.1) Molderings, G.J., et al. Immunogenetics 62 (11-12), 721-727 (2010) : |
| Immunogen | This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-836 amino acids from the C-terminal region of human KIT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Kit |
|---|---|
| Alternative Names | Mast/stem cell growth factor receptor Kit SCFR Proto-oncogene c-Kit Tyrosine-protein kinase Kit CD117 Kit Sl |
| Molecular Weight(MW) | 109 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
