Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. KLF12 is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1.Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene.1) Suda, S., (2006) Biochem. Biophys. Res. Commun. 344 (1), 246-252.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KLF12.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
KLF12 antibody can be used for detection of KLF12 by ELISA at 1:1562500. KLF12 antibody can be used for detection of KLF12 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
KLF12 antibody can be used for detection of KLF12 by ELISA at 1:1562500. KLF12 antibody can be used for detection of KLF12 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KLF12 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.