Application
Immunohistochemistry of KLF17 in mouse liver tissue with KLG17 antibody at 5 μg/ml.Application
Western blot analysis of KLF17 in rat liver tissue lysate with KLF17 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | KLF17 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KLF17, also known as ZNF393, is a member of the Sp/KLF family of transcription factors (1,2). KLF17 binds to the promoter and negatively regulates the transcription of Id-1, a key metastasis regulator in breast cancer (3). Suppression of KLF17 promotes breast cancer cell invasion and epithelial-mesenchymal transition (3). Down-regulated KLF17 expression is also associated tumor invasion and poor prognosis in hepatocellular carcinoma, suggesting that the level of KLF17 expression can serve as a prognostic indicator (4).1) Yan W, Burns KH, Ma L, et al. Identification of Zfp393, a germ cell-specific gene encoding a novel zinc finger protein. Mech. Dev. 2002; 118:233-9. |
| Immunogen | KLF17 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KLF17. The immunogen is located within the last 50 amino acids of KLF17. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| KLF17 antibody can be used for detection of KLF17 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLF17 |
|---|---|
| Alternative Names | Kruppel-like factor 17 zinc finger protein 393 ZNF393 Zfp393 |
| Molecular Weight(MW) | Predicted: 28, 39, 43 kDa Observed: 38 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (81%) |
| Tissue Specificity | KLF17 antibody is human, mouse and rat reactive. At least two isoforms of KLF17 are known to exist; this antibody will only detect the large isoform. KLF17 antibody is predicted to not cross-react with other KLF proteins. |
Application
Immunohistochemistry of KLF17 in mouse liver tissue with KLG17 antibody at 5 μg/ml.
Application
Western blot analysis of KLF17 in rat liver tissue lysate with KLF17 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | KLF17 antibody can be used for detection of KLF17 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KLF17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | KLF17 antibody is supplied in PBS containing 0.02% sodium azide. |
