Application
Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 μg/mL.Application
Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 μg/mL.Application
Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | KLHL15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KLHL15 Antibody: KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like proteins have been implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL15 mRNA is widely expressed in many tissues and is thought to be involved in protein ubiquination and cytoskeleton organization.1) Yoshida K. Identification and characterization of a novel kelch-like gene KLHL15 in silico. Oncol. Rep.2005; 13:1133-7. |
| Immunogen | KLHL15 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human KLHL15. The immunogen is located within the last 50 amino acids of KLHL15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLHL15 |
|---|---|
| Alternative Names | KLHL15 Antibody: HEL-S-305 KIAA1677 Kelch-like protein 15 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 μg/mL.
Application
Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 μg/mL.
Application
Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
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| Application Notes | KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
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| Storage Instructions | KLHL15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KLHL15 Antibody is supplied in PBS containing 0.02% sodium azide. |
