Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
KLHL6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected KLHL6 protein (arrow).| Product Name | KLHL6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kelch-like protein 6, KLHL6 |
| Product description | Involved in B-lymphocyte antigen receptor signaling and germinal center formation1) Gupta-Rossi, N., et al. Mol. Immunol. 39(13):791-799(2003) |
| Immunogen | This KLHL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-571 amino acids from the C-terminal region of human KLHL6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLHL6 |
|---|---|
| Alternative Names | Kelch-like protein 6 KLHL6 |
| Molecular Weight(MW) | 70 kDa |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
KLHL6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected KLHL6 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
