Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of KLHL9 Antibody in mouse testis tissue lysates (35ug/lane)Application
KLHL9 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | KLHL9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kelch-like protein 9, KLHL9, KIAA1354 |
| Product description | KLHL9 is substrate-specific adapter for a CUL3-based E3 ubiquitin-protein ligase complex. Within this complex, it controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. |
| Immunogen | This KLHL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-326 amino acids from the Central region of human KLHL9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLHL9 |
|---|---|
| Alternative Names | Kelch-like protein 9 KLHL9 KIAA1354 |
| Molecular Weight(MW) | 69 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of KLHL9 Antibody in mouse testis tissue lysates (35ug/lane)
Application
KLHL9 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
