Application
Immunofluorescence of KLRA1 in Human Spleen cells with KLRA1 antibody at 20 μg/mL.Application
Immunohistochemistry of KLRA1 in human spleen tissue with KLRA1 antibody at 2.5 μg/mL.Application
Western blot analysis of KLRA1 in 293 cell lysate with KLRA1 antibody at (A) 1 μg/mL and (B) 2 μg/mL.| Product Name | KLRA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KLRA1 Antibody: KLRA1 (also known and Ly49L) is a member of the LY49 family of receptors Natural killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells. At least three isoforms of KLRA1 are known to exist.1) Makrigiannis AP, Etzler J, Winkler-Pickett R, et al. Identification of the Ly49L protein: evidence for activating counterparts to inhibitory Ly49 proteins. J. Leuk. Biol.2000; 68:765-71. |
| Immunogen | KLRA1 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human KLRA1. The immunogen is located within amino acids 190 - 240 of KLRA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLRA1 |
|---|---|
| Alternative Names | KLRA1 Antibody: Ly49 KLRA# KLRA1 LY49L Ly-49L |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunofluorescence of KLRA1 in Human Spleen cells with KLRA1 antibody at 20 μg/mL.
Application
Immunohistochemistry of KLRA1 in human spleen tissue with KLRA1 antibody at 2.5 μg/mL.
Application
Western blot analysis of KLRA1 in 293 cell lysate with KLRA1 antibody at (A) 1 μg/mL and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
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| Application Notes | KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KLRA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KLRA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
