Application
Immunofluorescence of KLRA1 in 293 cells with KLRA1 antibody at 20 μg/mL.Application
Immunocytochemistry of KLRA1 in 293 cells with KLRA1 antibody at 4 μg/mL.Application
Western blot analysis of KLRA1 in mouse spleen tissue lysate with KLRA1 antibody at (A) 1 μg/mL and (B) 2 μg/mL.| Product Name | KLRA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KLRA1 Antibody: KLRA1 (also known as Ly49L) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells.1) Makrigiannis AP, Etzler J, Winkler-Pickett R, et al. Identification of the Ly49L protein: evidence for activating counterparts to inhibitory Ly49 proteins. J. Leuk. Biol.2000; 68:765-71. |
| Immunogen | KLRA1 antibody was raised against a 13 amino acid synthetic peptide near the center of human KLRA1. The immunogen is located within amino acids 140 - 190 of KLRA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLRA1 |
|---|---|
| Alternative Names | KLRA1 Antibody: Ly49 KLRA# KLRA1 LY49L Ly-49L |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform. |
Application
Immunofluorescence of KLRA1 in 293 cells with KLRA1 antibody at 20 μg/mL.
Application
Immunocytochemistry of KLRA1 in 293 cells with KLRA1 antibody at 4 μg/mL.
Application
Western blot analysis of KLRA1 in mouse spleen tissue lysate with KLRA1 antibody at (A) 1 μg/mL and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KLRA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KLRA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
