Application
Immunohistochemistry of KLRA2 in mouse spleen tissue with KLRA2 antibody at 2.5 μg/mL.Application
Western blot analysis of KLRA2 in mouse spleen tissue lysate with KLRA2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | KLRA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KLRA2 Antibody: KLRA2 (also known as Ly49B) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA2 is thought to be an inhibitory receptor and associates with the protein tyrosine phosphatases SHP1, SHP2, and SHIP in transfected cells. This antibody is predicted to not cross-react with other LY49 receptors.1) Gosselin P, Lusignan Y, Brennan J, et al. The NK2.1 receptor is encoded by Ly-49C and its expression is regulated by MHC class I alleles. Int. Immunol.1997; 533-40. |
| Immunogen | KLRA2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of mouse KLRA2. The immunogen is located within the last 50 amino acids of KLRA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| KLRA2 antibody can be used for detection of KLRA2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Klra2 |
|---|---|
| Alternative Names | KLRA2 Antibody: Ly49b Klra30 Ly49-b Ly49b Killer cell lectin-like receptor 2 Lymphocyte antigen 49b Ly-49b |
Application
Immunohistochemistry of KLRA2 in mouse spleen tissue with KLRA2 antibody at 2.5 μg/mL.
Application
Western blot analysis of KLRA2 in mouse spleen tissue lysate with KLRA2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | KLRA2 antibody can be used for detection of KLRA2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KLRA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KLRA2 Antibody is supplied in PBS containing 0.02% sodium azide. |
