Application
Flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with KMO Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of KMO Antibody in CEM cell line lysates (35ug/lane)Application
Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.| Product Name | KMO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kynurenine 3-monooxygenase {ECO:0000255|HAMAP-Rule:MF_03018}, 114139 {ECO:0000255|HAMAP-Rule:MF_03018}, Kynurenine 3-hydroxylase {ECO:0000255|HAMAP-Rule:MF_03018}, KMO |
| Product description | Kynurenine 3-monooxygenase (KMO; EC 1.14.13.9) is an NADPH-dependent flavin monooxygenase that catalyzes the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine.1) Ligam,P., et.al., Placenta 26 (6), 498-504 (2005) |
| Immunogen | This KMO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-182 amino acids from the Central region of human KMO. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KMO |
|---|---|
| Alternative Names | Kynurenine 3-monooxygenase {ECO:0000255|HAMAP-Rule:MF_03018} 114139 {ECO:0000255|HAMAP-Rule:MF_03018} Kynurenine 3-hydroxylase {ECO:0000255|HAMAP-Rule:MF_03018} KMO |
| Molecular Weight(MW) | 56 kDa |
Application
Flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with KMO Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of KMO Antibody in CEM cell line lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
