Application
Immunofluorescence of KPNA4 in HeLa cells with KPNA4 antibody at 20 μg/mL.Application
Immunocytochemistry of KPNA4 in HeLa cells with KPNA4 antibody at 2.5 μg/mL.Application
Western blot analysis of KPNA4 in HeLa cell lysate with KPNA4 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | KPNA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA4 Antibody: QIP1, SRP3, IPOA3, QIP1, Importin subunit alpha-3, Importin alpha Q1, Qip1 |
| Product description | KPNA4 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. Molecules containing the classical nuclear localization signal (NLS) are transported into the nucleus by alpha/beta heterodimers. KPNA3 has been shown to be important in the TNF-α-induced nuclear import of NF-κB. It is also involved in the stress-mediated nuclear stabilization of p53 and the nuclear import and replication of HIV-1 in both dividing and non-dividing cells.1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72. |
| Immunogen | KPNA4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA4. The immunogen is located within amino acids 30 - 80 of KPNA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| KPNA4 antibody can be used for detection of KPNA4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA4 |
|---|---|
| Alternative Names | KPNA4 Antibody: QIP1 SRP3 IPOA3 QIP1 Importin subunit alpha-3 Importin alpha Q1 Qip1 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunofluorescence of KPNA4 in HeLa cells with KPNA4 antibody at 20 μg/mL.
Application
Immunocytochemistry of KPNA4 in HeLa cells with KPNA4 antibody at 2.5 μg/mL.
Application
Western blot analysis of KPNA4 in HeLa cell lysate with KPNA4 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | KPNA4 antibody can be used for detection of KPNA4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KPNA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KPNA4 Antibody is supplied in PBS containing 0.02% sodium azide. |
