Application
Immunofluorescence of KPNA6 in 293 cells with KPNA6 antibody at 20 μg/mL.Application
Western blot analysis of KPNA6 in 293 cell lysate with KPNA6 antibody at 1 μg/mL.| Product Name | KPNA6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA6 Antibody: IPOA7, KPNA7, IPOA7, Importin subunit alpha-7, Karyopherin subunit alpha-6 |
| Product description | KPNA6 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA6 belongs to a subfamily within the KPNA family that also includes IPOA5&6. Down-regulation of KPNA6 by RNAi expression in HeLa cells strongly inhibited cell proliferation, possibly due to blocking the nuclear import of specific factors essential for cell growth and proliferation. KPNA6 also interacts with the Rev protein of HIV-1 and promote its nuclear import.1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72. |
| Immunogen | KPNA6 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human KPNA6. The immunogen is located within amino acids 70 - 120 of KPNA6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| KPNA6 antibody can be used for detection of KPNA6 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA6 |
|---|---|
| Alternative Names | KPNA6 Antibody: IPOA7 KPNA7 IPOA7 Importin subunit alpha-7 Karyopherin subunit alpha-6 |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
Application
Immunofluorescence of KPNA6 in 293 cells with KPNA6 antibody at 20 μg/mL.
Application
Western blot analysis of KPNA6 in 293 cell lysate with KPNA6 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | KPNA6 antibody can be used for detection of KPNA6 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KPNA6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KPNA6 Antibody is supplied in PBS containing 0.02% sodium azide. |
