WB
Western blot analysis of lysates from HeLa cells using KPNA6 Rabbit mAb at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.ICC/IF
Confocal imaging of A-431 cells using KPNA6 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat AntiRabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with αTubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488- conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (blue). objective: 100x.| Product Name | KPNA6 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 69-169 of human KPNA6 (NP_036448.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:2000-1:20000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA6 |
|---|---|
| Gene Synonyms | IPOA7 |
| Gene Full Name | karyopherin subunit alpha 6 |
| Gene Summary | Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. [provided by RefSeq, Jul 2008] |
| Alternative Names | IPOA7 KPNA6 |
| Molecular Weight(MW) | 60kDa |
| Cellular Localization | cytosol,nucleoplasm,nucleus |
WB
Western blot analysis of lysates from HeLa cells using KPNA6 Rabbit mAb at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
ICC/IF
Confocal imaging of A-431 cells using KPNA6 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat AntiRabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with αTubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488- conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (blue). objective: 100x.| Application Notes | WB:1:2000-1:20000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
