Application
Western blot analysis of KREMEN1 in rat small intestine tissue lysate with KREMEN1 antibody at (A) 0.125 and (B) 0.25 μg/mLApplication
Immunofluorescence of KREMEN1 in human small intestine tissue with KREMEN1 antibody at 20 μg/ml.Application
Immunohistochemistry of KREMEN1 in human small intestine tissue with KREMEN1 antibody at 5 μg/ml.| Product Name | KREMEN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KREMEN1 Antibody: KRM1, KREMEN, KRM1, Kremen protein 1, Dickkopf receptor |
| Product description | KREMEN1 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) coreceptor. KREMEN1 is a receptor for the Dickkopf protein which blocks Wnt/beta catenin signaling. It is necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes.1) Nakamura T, Aoki S, Kitajima K, et al. Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein. Biochim. Biophys. Acta. 2001; 1518:63-72. |
| Immunogen | Rabbit polyclonal KREMEN1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human KREMEN1. The immunogen is located within the last 50 amino acids of KREMEN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| KREMEN1 antibody can be used for detection of KREMEN1 by Western blot at 0.125 - 0.25 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KREMEN1 |
|---|---|
| Alternative Names | KREMEN1 Antibody: KRM1 KREMEN KRM1 Kremen protein 1 Dickkopf receptor |
| Molecular Weight(MW) | 54 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | Three isoforms of KREMEN1 exists as a result of alternative splicing event. |
Application
Western blot analysis of KREMEN1 in rat small intestine tissue lysate with KREMEN1 antibody at (A) 0.125 and (B) 0.25 μg/mL
Application
Immunofluorescence of KREMEN1 in human small intestine tissue with KREMEN1 antibody at 20 μg/ml.
Application
Immunohistochemistry of KREMEN1 in human small intestine tissue with KREMEN1 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | KREMEN1 antibody can be used for detection of KREMEN1 by Western blot at 0.125 - 0.25 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KREMEN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | KREMEN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
