Application
Western blot analysis of KREMEN2 in HeLa cell lysate with KREMEN2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of KREMEN2 in HeLa cells with KREMEN2 antibody at 5 μg/ml.| Product Name | KREMEN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KREMEN2 Antibody: KRM2, KRM2, Kremen protein 2, Dickkopf receptor 2 |
| Product description | KREMEN2 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.1) Nakamura T, Aoki S, Kitajima K, et al. Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein. Biochim. Biophys. Acta. 2001; 1518:63-72. |
| Immunogen | KREMEN2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human KREMEN2 . The immunogen is located within amino acids 390 - 440 of KREMEN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| KREMEN2 Antibody can be used for detection of KREMEN2 by Western blot starting at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KREMEN2 |
|---|---|
| Alternative Names | KREMEN2 Antibody: KRM2 KRM2 Kremen protein 2 Dickkopf receptor 2 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 54 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members. |
Application
Western blot analysis of KREMEN2 in HeLa cell lysate with KREMEN2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of KREMEN2 in HeLa cells with KREMEN2 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | KREMEN2 Antibody can be used for detection of KREMEN2 by Western blot starting at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KREMEN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | KREMEN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
