Application
Immunohistochemistry staining of KRT19 in uterus tissue using KRT19 monoclonal Antibody.| Product Name | KRT19 Antibody, Monoclonal |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT19, CK19, Cytokeratin 19, Cytokeratin-19, Keratin 19, Keratin-19, CK-19, K19, K1CS, Keratin, type I, 40-kd |
| Product description | Keratin, Type I Cytoskeletal 19 (KRT19) Mouse anti-Human Monoclonal (0.N.365) Antibody. Also available without BSA and azide. Immunofluorescence, Western Blotting, Immunohistochemistry (frozen and formalin/paraffin). Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1 mg/mL Tris-HCl, pH 2.0 for 10-15 min at RT; with trypsin at 1 mg/mL Tris-HCl (or PBS), pH 7.4 for 10-15 min at RT); or by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min., followed by cooling at RT for 20 min. Protease XXIV damages the epitope recognized by this antibody. Recognizes the rod domain of keratin 19 (40 kDa). Species Reactivity: Human. Weak reactivity with Rat, Mouse and Guinea pig. |
| Immunogen | KRT19 monoclonal antibody was raised against amino acids from the C-Terminus of KRT19 (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,l |
| Host Species | Mouse |
| Tested Applications | IFIHCWB |
| KRT19 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 10 μg/mL.: |
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| Species Reactivity | Guinea PigHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | KRT19 |
|---|---|
| Alternative Names | KRT19 CK19 Cytokeratin 19 Cytokeratin-19 Keratin 19 Keratin-19 CK-19 K19 K1CS Keratin type I 40-kd |
| Tissue Specificity | Recognizes the rod domain of keratin 19 (40kD). Reacts with the MCF-7 cells, which are known to contain keratin 19. It shows no reaction with the cells lacking keratin 19 such as A431 and HeLa. |
Application
Immunohistochemistry staining of KRT19 in uterus tissue using KRT19 monoclonal Antibody.| Application Notes | KRT19 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 10 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store KRT19 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |
