Application
Immunochemistry staining of TPA - induced BCBL - 1 cells using monoclonal antibody to KSHV K8a. HRP - anti - mouse was used as the second antibody before color development with AEC.Application
Western blot analysis using KSHV K8alpha monoclonal antibody against TPA induced BCBL - 1 cell lysate.| Product Name | KSHVK8a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 SUMO-protein ligase K-bZIP |
| Product description | Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. KSHV K8 and K8.1 open reading frames are juxtaposed and span from nucleotide (nt) 74850 to 76695 of the virus genome. A K8 pre-mRNA overlaps the entire K8.1 coding region, and alternative splicing of KSHV K8 and K8.1 pre-mRNAs each produces three isoforms (α,β, and γ) of the mRNAs.1) James J et al. Proc. Natl. Acad. Sci Vol. 1996. 93, pp. 14862-14867. |
| Immunogen | Ni-NTA purified recombinant human KSHV K8α expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICCWB |
| Western Blot:Dilution 1:500 - 1,000 ICC:Dilution 1:50 - 1:100 Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Gene Symbol | K8 |
|---|---|
| Alternative Names | E3 SUMO-protein ligase K-bZIP |
Application
Immunochemistry staining of TPA - induced BCBL - 1 cells using monoclonal antibody to KSHV K8a. HRP - anti - mouse was used as the second antibody before color development with AEC.
Application
Western blot analysis using KSHV K8alpha monoclonal antibody against TPA induced BCBL - 1 cell lysate.| Application Notes | Western Blot:Dilution 1:500 - 1,000 ICC:Dilution 1:50 - 1:100 Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | KSHV K8α monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
