Application
Western blot analysis of L1TD1 in Jurkat cell lysate with L1TD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | L1TD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | L1TD1 Antibody: ECAT11, ECAT11, LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11 |
| Product description | L1TD1 Antibody: L1TD1 (ECAT11) is a member of the family of Embryonic Stem Cell Associated Transcripts (ECAT) that play an important role in stem cell biology. ECAT4 (Nanog) is a master regulator for the maintenance of mESC and hESC. ECAT5 was identified to be Embryonic stem cell expressed Ras (ERas), a Ras-like oncogene that is important in regulating mESC proliferation. ECAT9 (GDF3) is an important factor that helps maintain mESC pluripotency by inhibiting bone morphogenetic protein (BMP) signaling. L1TD1 is highly enriched in undifferentiated hESC and L1TD1 is a target gene of Nanog.1) Iwabuchi KA, Yamakawa T, Sato Y, et al. ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency. PLoS One. 2011; 6:e20461. |
| Immunogen | L1TD1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human L1TD1. The immunogen is located within amino acids 690 - 740 of L1TD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| L1TD1 antibody can be used for detection of L1TD1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | L1TD1 |
|---|---|
| Alternative Names | L1TD1 Antibody: ECAT11 ECAT11 LINE-1 type transposase domain-containing protein 1 ES cell-associated protein 11 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (76%) |
| Tissue Specificity | L1TD1 antibody is predicted to not cross-react with other ECAT protein family members |
Application
Western blot analysis of L1TD1 in Jurkat cell lysate with L1TD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | L1TD1 antibody can be used for detection of L1TD1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | L1TD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | L1TD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
