Application
Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 μg/mL.| Product Name | LAMP-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMP-2 Antibody: Mac3, LGP-B, CD107b, Lamp-2, Lamp II, Lamp-2a, Lamp-2b, Lamp-2c, Lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B, LAMP-2 |
| Product description | LAMP-2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906. |
| Immunogen | LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2. The immunogen is located within the last 50 amino acids of LAMP-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Lamp2 |
|---|---|
| Alternative Names | LAMP-2 Antibody: Mac3 LGP-B CD107b Lamp-2 Lamp II Lamp-2a Lamp-2b Lamp-2c Lysosome-associated membrane glycoprotein 2 CD107 antigen-like family member B LAMP-2 |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
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| Application Notes | LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LAMP-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LAMP-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
