Application
Immunohistochemistry of Lano in human colon tissue with Lano antibody at 10 μg/mL.Application
Immunofluorescence of Lano in human colon tissue with Lano antibody at 20 μg/mL.Application
Western blot analysis of Lano in PC-3 whole cell lysate with Lano antibody at (A) 1 or (B) 2 μg /ml.| Product Name | Lano Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lano Antibody: LANO, dJ523E19.1, LANO, Leucine-rich repeat-containing protein 1, LANO adapter protein |
| Product description | Lano Antibody: Lano is a member of the LAP (leucine-rich repeats and PDZ) family of proteins that also includes Densin-180, Erbin, and hScribble. The LAP proteins generally contain multiple leucine-rich repeat (LRR) domains which serve to target them to the basolateral membrane of epithelial cells. Lano is unique in that it alone does not possess one or more PDZ (PSD95/DLG/ZO-1) domains as do the other members of the LAP family. However, it can bind to the PDZ domain of Erbin in addition to those of membrane-associated and guanylate kinase (MAGUK) proteins which regulate adhesion and plasticity at cell junctions. It has been suggested that it is through these interaction that these LAP proteins participate in the maintenance of proper embryonic development and integrity of epithelial tissues.1) Saito H, Santoni M-J, Jaulin-Bastard F, et al. Lano, a novel LAP protein directly connected to MAGUK proteins in epithelial cells. J. Biol. Chem. 2001; 276:32051-5. |
| Immunogen | Lano antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Lano. The immunogen is located within the last 50 amino acids of Lano. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Lano antibody can be used for detection of Lano by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRRC1 |
|---|---|
| Alternative Names | Lano Antibody: LANO dJ523E19.1 LANO Leucine-rich repeat-containing protein 1 LANO adapter protein |
| Sequence Similarities | 2) Cat. No. 1320 - Human Colon Tissue Lysate |
Application
Immunohistochemistry of Lano in human colon tissue with Lano antibody at 10 μg/mL.
Application
Immunofluorescence of Lano in human colon tissue with Lano antibody at 20 μg/mL.
Application
Western blot analysis of Lano in PC-3 whole cell lysate with Lano antibody at (A) 1 or (B) 2 μg /ml.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | Lano antibody can be used for detection of Lano by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Lano antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Lano Antibody is supplied in PBS containing 0.02% sodium azide. |
