Application
Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | LARGE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glycosyltransferase-like protein LARGE1, 2.4.-.-, Acetylglucosaminyltransferase-like 1A, Xylosyltransferase LARGE, 2.4.2.-, Beta-1,3-glucuronyltransferase LARGE, 2.4.1.-, LARGE, KIAA0609, LARGE1 |
| Product description | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine- beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed: 22223806 ). Phosphorylated O- mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed: 25279699 ).1) Peyrard M.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:598-603(1999). |
| Immunogen | This LARGE antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 365-398 amino acids from the Central region of human LARGE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LARGE |
|---|---|
| Alternative Names | Glycosyltransferase-like protein LARGE1 2.4.-.- Acetylglucosaminyltransferase-like 1A Xylosyltransferase LARGE 2.4.2.- Beta-1 3-glucuronyltransferase LARGE 2.4.1.- LARGE KIAA0609 LARGE1 |
| Molecular Weight(MW) | 88 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
